منابع مشابه
Inhibition by Vanadate of the Reactions Catalyzed stimulated ATPase
The interaction of vanadate with the (Na' + K')st imulated ATPase from electric organ was investigated using the acid quench-flow technique. A t 21"C, incubation of the enzyme with 1.3 to 1.6 p~ vanadate in the presence of 75 m~ Na' and 25 mM K' strongly inhibits phosphorylation by ATP. Enzyme activity remaining under these conditions shows no change in the apparent rates of phosphorylation or...
متن کاملPlasma Membrane-stimulated Vanadate-dependent NADH Oxidation Is Not the Primary Mediator of Vanadate Toxicity in Succharomyces
Interactions of oxyvanadium compounds with cellular metabolism have recently been demonstrated. Membrane-stimulated vanadate-dependent NADH oxidation has been hypothesized to involve the cellular accumulation of HzOz, which may cause the vanadate sensitivity of animals and microbes. This report shows that the vanadate-dependent NADH oxidation activity of the yeast plasma membrane requires oxyge...
متن کاملCharacterization of vanadate-dependent NADH oxidation stimulated by Saccharomyces cerevisiae plasma membranes.
Plasma membrane-stimulated vanadate-dependent NADH oxidation has been characterized in Saccharomyces cerevisiae. This activity is specific for vanadate, because molybdate, a similar metal oxide, did not substitute for vanadate in the reaction. Vanadate-dependent plasma membrane-stimulated NADH oxidation activity was dependent on the concentrations of vanadate, NADH, and NADPH and required funct...
متن کاملThe effect of vanadate upon calcium-stimulated ATPase of the rabbit iris-ciliary body.
In many tissues, the level of cytoplasmic calcium mediates cell function. Since cytoplasmic calcium is often maintained at a low level by active calcium extrusion, we examined whether calcium-stimulated ATPase is present in the rabbit ciliary epithelium. A technique was developed to measure calcium-stimulated ATPase in a partially enriched plasma membrane preparation. The enhancement of Na,K-AT...
متن کاملEffect of sodium vanadate
Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, AGEPC) and sodium vanadate (a phosphotyrosine phosphatase inhibitor) induced a timeand concentration-dependent increase in phosphotyrosine in several proteins and stimulated prostaglandin (PG) E2 production in cultured rat Kupffer cells. In addition, vanadate induced a decrease in the surface expression of AGEPC recepto...
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ژورنال
عنوان ژورنال: Nature
سال: 1978
ISSN: 0028-0836,1476-4687
DOI: 10.1038/275768a0